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    <default:controlfield tag="008">       19uu9999   |||||s|||||00| 0|eng d </default:controlfield>
				
    <default:datafield ind1="1" ind2=" " tag="700">
		    			
      <default:subfield code="a">Buckle Ashley M</default:subfield>
				
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    <default:datafield ind1="1" ind2=" " tag="700">
		    			
      <default:subfield code="a">Law Ruby H. P.</default:subfield>
				
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    <default:datafield ind1="1" ind2=" " tag="700">
		    			
      <default:subfield code="a">Whisstock James C.</default:subfield>
				
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    <default:datafield ind1="1" ind2=" " tag="700">
		    			
      <default:subfield code="a">Dunstone Michelle A.</default:subfield>
				
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    <default:datafield ind1="0" ind2="0" tag="245">
						
      <default:subfield code="a">2QP2 - Structure of a MACPF/Perforin-like protein</default:subfield>
				
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      <default:subfield code="a">Protein crystallography raw diffraction images and unmerged reflection intensities Collection size: 36.1 GB Number of datasets: 5 Citation: Rosado et. al. (2007) A common fold mediates vertebrate defense and bacterial attack. Science. In Press.
Proteins containing membrane attack complex/perforin (MACPF) domains play important roles in vertebrate immunity, embryonic development, and neural-cell migration. In vertebrates, the ninth component of complement and perforin form oligomeric pores that lyse bacteria and kill virus-infected cells, respectively. However, the mechanism of MACPF function is unknown. We determined the crystal structure of a bacterial MACPF protein, Plu-MACPF from Photorhabdus luminescens, to 2.0 angstrom resolution. The MACPF domain reveals structural similarity with poreforming cholesterol-dependent cytolysins (CDCs) from Gram-positive bacteria. This suggests that lytic MACPF proteins may use a CDC-like mechanism to form pores and disrupt cell membranes. Sequence similarity between bacterial and vertebrate MACPF domains suggests that the fold of the CDCs, a family of proteins important for bacterial pathogenesis, is probably used by vertebrates for defense against infection.</default:subfield>
				
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      <default:subfield code="a">data holding</default:subfield>
				
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    <default:datafield ind2="0" tag="655">
						
      <default:subfield code="a">crystal structure data holding</default:subfield>
				
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    <default:datafield ind1="0" ind2="0" tag="773">
						
      <default:subfield code="t">http://www.sciencemag.org/cgi/content/abstract/1144706</default:subfield>
				
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